Panel A shows representative single-channel currents for various ELIC single/double mutants. The x-axis represents time in seconds, and the y-axis represents current in picoamperes. Panel B presents current-voltage relationships for the mutants described in Panel A, using the same color scheme. The x-axis represents holding potential in millivolts, and the y-axis represents current in picoamperes. Data points from individual recordings are shown as dots, with the mean represented by diamonds and the standard deviation indicated by crossbars. The graphs illustrate how different mutations affect the single-channel conductance of ELIC.
Mutations of acid residues in the lateral pathway reduce single-channel conductance. (A) Representative single-channel currents of ELIC single/double mutants (made on a WT background). Data for WT ELIC from Fig. 2 C are shown in black for reference. Mutants of the apical barrier residues are shown in blue (R65A) and green (R65A/K90A), and mutants of the lateral fenestration residues are shown in gold (D113A/D158A) and purple (E30A/D158A). (B) I–V relationships for the mutants described in A are shown with the same color scheme. The data from individual recordings is shown as dots with the mean as diamonds and the standard deviation as crossbars. Slope conductances were calculated separately for positive and negative potentials for each mutant. The slope conductances are not displayed here for clarity but are available in Table 3 along with the rectification ratio. The slope conductances are calculated using the dominant single-channel currents from each patch.