Figure S7.
The left graph shows net outward sodium ion permeation events as a function of time. The x-axis is labeled Time in nanoseconds, ranging from 0 to 150 nanoseconds, and the y-axis is labeled Net Outward Sodium Ion Permeation Events, ranging from minus 40 to 40. Different lines represent various applied potentials: minus 250 millivolts (dark red), minus 150 millivolts (red), minus 100 millivolts (light red), 0 millivolts (gray), 100 millivolts (light blue), 150 millivolts (blue), and 250 millivolts (dark blue). The right graph shows net outward chloride ion permeation events as a function of time. The x-axis is labeled Time in nanoseconds, ranging from 0 to 150 nanoseconds, and the y-axis is labeled Net Outward Chloride Ion Permeation Events, ranging from minus 10 to 0. The lines in this graph represent the same applied potentials as in the left graph. The left graph shows that as the applied potential increases, the net outward sodium ion permeation events increase over time, whereas negative potentials result in a decrease in net outward sodium ion permeation events. The right graph indicates that net outward chloride ion permeation events remain generally negative, suggesting a net inward movement of chloride ions, with slight variations over time depending on the applied potential.
Net outward permeations events for the ELIC5 MD refined structure 2. Plots of the net outward permeation events for Na+ (left) and Cl− (right) ions through the ELIC5 MD refined structure 2 are shown as a function of time. All independent replicates (n = 3) are shown for all potentials (−250 mV, dark red; −150 mV, red; −100 mV, light red; 0 mV, gray; +100 mV, light blue; +150 mV, blue; +250 mV, dark blue). Ions traversing the channel from intracellular side to extracellular side are noted as +1 outward permeation events. Ions traversing the channel from extracellular side to intracellular side are noted as −1 outward permeation events. These ion counts are associated with Fig. 6 (bottom).