Figure S6.
The left graph shows the net outward sodium ion permeation events over time for the ELIC5 MD refined Structure 1. The x-axis represents time in nanoseconds, ranging from 0 to 150 nanoseconds, and the y-axis represents the net outward sodium ion permeation events, ranging from minus 20 to 40. The graph includes multiple lines representing different applied potentials: minus 250 millivolts (dark red), minus 100 millivolts (light red), 0 millivolts (gray), 100 millivolts (light blue), and 250 millivolts (dark blue). Each line shows the net outward permeation events for sodium ions, with positive values indicating ions moving from the intracellular side to the extracellular side and negative values indicating the opposite direction. The right graph shows the net outward chloride ion permeation events over time for the same structure. The x-axis represents time in nanoseconds, ranging from 0 to 150 nanoseconds, and the y-axis represents the net outward chloride ion permeation events, ranging from minus 10 to 0. The graph shows a single gray line representing the net outward permeation events for chloride ions, with negative values indicating ions moving from the extracellular side to the intracellular side. The graphs illustrate the time-dependent behavior of ion permeation events under different applied potentials.
Net outward permeations events for the ELIC5 MD refined structure 1. Plots of the net outward permeation events for Na+ (left) and Cl− (right) ions through the ELIC5 MD refined structure 1 are shown as a function of time. All independent replicates (n = 3) are shown for all potentials (−250 mV, dark red; −100 mV, light red; 0 mV, gray; +100 mV, light blue; +250 mV, dark blue). Ions traversing the channel from intracellular side to extracellular side are noted as +1 outward permeation events. Ions traversing the channel from extracellular side to intracellular side are noted as −1 outward permeation events. These ion counts are associated with Fig. 6 (top).