Figure S3.
Two step-line plots show simulation data for the alpha 1 glycine receptor channel over time. Left plot: The y-axis is labeled Net Outward Sodium ion Permeation Events, ranging from negative 10 to 0. The x-axis is labeled Time in nanoseconds, ranging from 0 to 150. The blue trace stays flat at 0 throughout. The red trace steps downward in stages: dropping to about negative 2 around 15 nanoseconds, back up to negative 1 near 45 nanoseconds, down again to negative 2 by 60 nanoseconds, holding until around 110 nanoseconds, then stepping further down to negative 4, briefly to negative 2, and finally settling around negative 3 to negative 4 by 150 nanoseconds. Right plot: The y-axis is labeled Net Outward Chloride ion Permeation Events, ranging from negative 40 to 40. The x-axis is labeled Time in nanoseconds, ranging from 0 to 150. Multiple red traces rise steadily in a stepwise pattern from around 0 at 10 nanoseconds to approximately 40 to 48 events by 150 nanoseconds. Multiple blue traces decline gradually in a stepwise pattern from around 0 to approximately negative 5 to negative 8 by 150 nanoseconds. All data are approximate.
Net outward permeations events for the purported open structure of zebrafish α1 GlyR. Plots of the net outward permeation events for Na+ (left) and Cl− (right) ions through the purported open structure of zebrafish α1 GlyR are shown as a function of time. All independent replicates (n = 3) are shown for all potentials (−250 mV, dark red; +250 mV, dark blue). Ions traversing the channel from intracellular side to extracellular side are noted as +1 outward permeation events. Ions traversing the channel from extracellular side to intracellular side are noted as −1 outward permeation events. These ion counts are associated with Fig. 2 E.