Figure S2.
In the left plot, the y-axis is labeled Net Outward Sodium ion Permeation Events, ranging from negative 30 to 40. The x-axis is labeled Time in nanoseconds, ranging from 0 to 150. Two conditions are plotted, based on Applied Potential in millivolts: negative 250 millivolts in red and 250 millivolts in blue. The red traces, negative 250 millivolts, stay near zero, drifting slightly downward to about negative 3 by 150 nanoseconds. The blue traces, 250 millivolts, rise in a stepwise pattern starting around 15 nanoseconds, climbing steadily to reach approximately 20 to 26 events by 150 nanoseconds, with two blue traces shown following similar upward paths. Right plot: The y-axis is labeled Net Outward Chloride ion Permeation Events, ranging from negative 10 to 0. The x-axis is labeled Time in nanoseconds, ranging from 0 to 150. Most traces stay flat at 0 throughout. One trace steps downward in stages starting around 45 nanoseconds, dropping to about negative 1, then negative 2, then negative 3 around 60 to 80 nanoseconds, continuing to step down further to reach about negative 8 by 150 nanoseconds. All data are approximate.
Net outward permeations events for the purported open structure of human α7 nAChR. Plots of the net outward permeation events for Na+ (left) and Cl− (right) ions through the purported open structure of human α7 nAChR are shown as a function of time. All independent replicates (n = 3) are shown for all potentials (−250 mV, dark red; +250 mV, dark blue). Ions traversing the channel from intracellular side to extracellular side are noted as +1 outward permeation events. Ions traversing the channel from extracellular side to intracellular side are noted as −1 outward permeation events. These ion counts are associated with Fig. 2 D.