Figure S1.
The left graph shows net outward sodium ion permeation events, while the right graph shows net outward chloride ion permeation events. Both graphs have time (nanoseconds) on the horizontal axis ranging from 0 to 150 nanoseconds. The vertical axis of the left graph measures net outward sodium ion permeation events ranging from minus 40 to 40, and the vertical axis of the right graph measures net outward chloride ion permeation events ranging from minus 10 to 0. The left graph includes multiple lines representing different applied potentials: minus 250 millivolts (dark red), minus 100 millivolts (light red), 0 millivolts (gray), positive 100 millivolts (light blue), and positive 250 millivolts (dark blue). The right graph shows a single line for 0 millivolts (gray). In the left graph, the net outward sodium ion permeation events increase over time for positive potentials (positive 100 millivolts and positive 250 millivolts) and decrease for negative potentials (minus 250 millivolts and minus 100 millivolts). The 0 millivolt line remains relatively flat. The right graph shows no significant change in net outward chloride ion permeation events over time.
Net outward permeations events for the ELIC5 cryo-EM structure. Plots of the net outward permeation events for Na+ (left) and Cl− (right) ions through the ELIC5 cryo-EM structure are shown as a function of time. All independent replicates (n = 3) are shown for all potentials (−250 mV, dark red; −100 mV, light red; 0 mV, gray; +100 mV, light blue; +250 mV, dark blue). Ions traversing the channel from intracellular side to extracellular side are noted as +1 outward permeation events. Ions traversing the channel from extracellular side to intracellular side are noted as −1 outward permeation events. These ion counts are associated with Fig. 2 B.