Panel A shows the baseline Fura ratio (340/380) at diastole, with the y-axis labeled Baseline Fura [340/380] and the x-axis labeled with categories: Sex (M, F) and HFpEF (minus, plus). Panel B displays the amplitude of the Ca2 plus transient, with the y-axis labeled Amplitude [340/380] and the x-axis labeled similarly. Panel C illustrates the time to peak of the Ca2 plus transient, with the y-axis labeled Time to Peak [milliseconds] and the x-axis labeled with the same categories. Panel D shows the time to 50 percent transient decay (RT50), with the y-axis labeled RT50 [milliseconds] and the x-axis labeled similarly. Each panel includes data points for male-ctrl, male HFpEF-like, female-ctrl, and female HFpEF-like groups. Significant p-values are indicated for specific comparisons, highlighting differences in Ca2 plus handling due to sex and heart condition.
Ca 2+ handling is altered in cardiomyocytes from male HFpEF-like mice. (A–D) Ca2+ transients in isolated intact cardiomyocytes were measured using Fura-2 under 4 Hz stimulation. The Fura ratio (340/380) at diastole (A), transient amplitude (B), time to peak (C), and time to 50% transient decay (RT50; D) are shown. The data are presented as means ± SE. The sample sizes include n = 334, 337, 226, and 483 cells from 7, 8, 7, and 10 mice for male-ctrl, male HFpEF-like, female-ctrl, and female HFpEF-like. Analysis was performed using a linear mixed model with least significant difference post hoc comparisons. Main effects are shown only when they are statistically significant. Each data point represents the average value of one mouse. For simplicity, post hoc comparisons between male-ctrl vs. female HFpEF-like and male HFpEF-like vs. female-ctrl are not shown.