Panel A shows data for male control mice, Panel B for male HFpEF-like mice, Panel C for female control mice, and Panel D for female HFpEF-like mice. Each graph has the x-axis labeled Sarcomere Length [micrometers] ranging from 1.8 to 2.2 and the y-axis labeled Diastolic Stress [millinewtons per square millimeter] ranging from 0.0 to 9.0. The data series are represented by different symbols and lines: open circles for DMSO before BDM, open diamonds for DMSO after BDM, filled circles for colchicine before BDM, and filled diamonds for colchicine after BDM. The graphs show the diastolic stress measurements before and after treatment with BDM, a crossbridge inhibitor, in colchicine-treated and DMSO-treated cardiomyocytes. No statistically significant effect of colchicine on diastolic stress is observed in any group, although the male HFpEF-like group shows a trend toward reduced diastolic stress before BDM treatment in colchicine compared to DMSO-treated cells. The data are presented as means with standard error bars.
Contribution of microtubules in intact cardiomyocytes with and without XB inhibition. (A–D) To evaluate the contribution of the microtubular network, diastolic stress–SL relation was measured in colchicine-treated and DMSO-treated cardiomyocytes, both before and after treatment with BDM, a XB inhibitor in (A) male-ctrl, (B) male HFpEF-like, (C) female-ctrl, and (D) female HFpEF-like mice. No statistically significant effect of colchicine on diastolic stress is observed in any group, although the male HFpEF-like group shows a trend toward reduced diastolic stress before BDM treatment in colchicine compared with DMSO-treated cells. The data are presented as means ± SE. The sample sizes are 9, 10, 8, and 11 mice for male-ctrl, male HFpEF-like, female-ctrl, and female HFpEF-like, with 2–4 colchicine-treated cells and 2–4 DMSO-treated cells analyzed per mouse. Analysis was performed using a linear mixed model at individual SLs. The statistical significance of stress before and after BDM is not shown for simplicity.