Figure S2.
Panel A contains a series of flow cytometric contour plots illustrating the gating strategies for various immune cell populations. The plots show the identification of leukocytes (CD45 plus), live cells, plasma cells (CD138 plus), B cells (CD19 plus), T cells (CD3 plus), CD4 T cells, Th17 cells (RORγt plus), Tregs (Foxp3 plus), macrophages (F4/80 plus), M1 macrophages, and M2 macrophages (CD206 plus). Each plot is labeled with specific markers used for gating, such as SSC-A, FSC-A, CD4, RORγt, Foxp3, CD138, CD19, CD3, F4/80, and CD206. The plots show the distribution and frequency of these cell populations. Panel B contains bar graphs summarizing the results of the flow cytometric analysis. The bar graphs compare the percentages of CD4 T cells, RORγt plus cells, Foxp3 plus cells, CD19 plus cells, CD138 plus cells, F4/80 plus cells, and CD206 plus cells between sham and Bb-infected groups. The y-axes represent the percentage of cells, and the x-axes represent the different experimental groups. The bars indicate the mean with standard error of the mean (SEM), and each symbol represents an individual mouse. The data show no significant differences in the frequencies of B cells, T cells, Th17 cells, and Tregs between the sham and Bb-infected groups. However, there are significant reductions in plasma cell frequencies and increases in total macrophage populations, with macrophages skewing toward a CD206 plus CD11c low M2-like phenotype in Bb-infected mice.
Phenotypic characterization of lamina propria immune cell populations following Bb infection. (A) Representative flow cytometric contour plots with outliers illustrating the gating for Th17 cells, Tregs, plasma cells, and M1/M2 macrophages. (B) Summary of the results. Bars indicate the mean ± SEM, and each symbol represents an individual mouse (n = 5 per group). Data are representative of pooled data from two independent experiments. Statistical analyses were conducted by the Mann–Whitney U test. ns, not significant (P > 0.05).