Confined CD36 ^WT tracks tend to be in locations with higher-than-average cortical actin intensity, while confined CD36 ^G12V trajectories do not. (A and B) Representative live cell images of confined single-molecule trajectories of CD36^WT (A) or of CD36^G12V (B) overlaid on SiR-actin–labeled actin cytoskeleton. Scale bar, 5 μm. For visual clarity, only confined tracks are shown, while all other types (free, immobile, directed, or too short to classify) are not. (C and D) Actin intensity (in arbitrary units [A.U.]) measured at confined CD36 tracks or in the whole cell (i.e., average) for CD36^WT (C) or CD36^G12V (D). Lines connect paired measurements from individual cells, comparing actin intensity measured at confined tracks to whole cell actin intensity. Red/blue lines indicate local enrichment/depletion at confined tracks relative to whole cell levels. Paired, right-tailed Wilcoxon rank-sum test comparing the actin intensity at confined tracks vs. whole cell levels yields P = 0.0873 for CD36^WT and P = 0.3706 for CD36^G12V. The right y-axes show effect size (Cohen’s d) of local actin intensity relative to whole cell measurements. The error bar shows the 95% confidence interval obtained from 3,000 bootstrap samples. The 95% confidence interval of CD36^WT does not cross zero (dashed gray line), indicating a significant difference between local and whole cell, while the 95% confidence interval of CD36^G12V crosses zero, indicating a lack of difference between local and whole cell. N = 28 cells from three experimental repeats in C and 28 cells from two experimental repeats in D.