Panel A shows boxplots with individual cell data points, mean of group of cells, standard deviation of group of cells, and the fraction of CD36WT and CD36G12V tracklets undergoing different motion types (Free, Confined, Immobile, Directed) in the whole imaged region. The x-axis represents the motion types, and the y-axis represents the fraction of diffusion type. Panel B and C show boxplots for the fraction of confined diffusion in the center and periphery regions, respectively. The x-axis represents CD36WT and CD36G12V, and the y-axis represents the fraction of confined diffusion. Panel D shows boxplots for the MSS slope for free and confined CD36WT and CD36G12V in the whole imaged region. The x-axis represents the motion types, and the y-axis represents the MSS slope. Panel E and F show boxplots for the diffusion coefficient and diffusion radius for free and confined CD36WT and CD36G12V tracklets in the whole imaged region. The x-axis represents the motion types, and the y-axis represents the diffusion coefficient and diffusion radius, respectively. Panel G shows cumulative distribution functions for the SM diffusion coefficient as input into simulations, as measured for simulated free and confined tracks, and as measured experimentally. The x-axis represents the SM diffusion coefficient, and the y-axis represents the cumulative distribution function. Panel H shows boxplots for the strength of confinement derived from the scaling exponent of the mean squared displacement. The x-axis represents CD36WT and CD36G12V, and the y-axis represents the strength of confinement. The graphs collectively illustrate that the G12V mutation increases the fraction of CD36 molecules undergoing confined diffusion but with weaker confinement strength.
G12V mutation increases fraction of CD36 molecules undergoing confined diffusion, but with weaker confinement strength. (A) Fraction of CD36WT and CD36G12V tracklets undergoing the indicated motion types in the whole imaged region. (B and C) Fraction of CD36WT and CD36G12V tracklets undergoing confined diffusion in the center (B) and periphery (C) regions. (D) MSS slope for free and confined CD36WT and CD36G12V in the whole imaged region. (E and F) Diffusion coefficient (E) and diffusion radius (F) for free and confined CD36WT and CD36G12V tracklets in the whole imaged region. (G) The cumulative distribution of SM diffusion coefficient as input into simulations (solid gray lines), as measured for simulated free and confined tracks (dashed lines), and as measured experimentally (solid non-gray lines). The red box zooms in on an area for visual aid to distinguish between highly overlapping lines. (H) The strength of confinement as derived from the scaling exponent of the mean squared displacement. In all panels but G, circles, red lines, shaded bars, and asterisks (or lack thereof) are as in Fig. 2. See Table 5 for number of cells, number of experimental repeats, and number of SM tracklets and speckles per ROI used for analysis.