G12V mutation reduces coupling between CD36 mobility type and local CA speckle displacement and redistributes CD36 from periphery to center. (A and B) Regression coefficients of CD36^WT and CD36^G12V MSS slope on local CA speckle displacement, from MVRG analysis on speckle displacement and density (see Fig. S6 for corresponding regression coefficients on density). MVRG analysis was performed separately for the four indicated subgroups of SM tracklets based on subcellular region (center [A] and periphery [B]) combined with local CA speckle density. Circles indicate individual cell measurements; gray circles indicate inliers while non-gray circles indicate outliers. Red lines and shaded bars show mean and standard deviation, respectively, over a group of cells representing WT or mutant. MVRG coefficients significantly different from zero (dashed gray line) are indicated by colored asterisks above the MVRG coefficient plots. Significant differences between WT and mutant are indicated by black asterisks above the bracket. For both, *, **, and *** indicate P values ≤ 0.05, 0.01, and 0.001, respectively. In all tests, P values > 0.05 are not explicitly indicated to reduce clutter. Inset pictograms: visual summary of MVRG results. Triangle slope and direction represent significance and sign (positive or negative) of MVRG coefficient; rectangles indicate nonsignificant MVRG coefficients. (C) SM tracklet density for WT and mutant CD36 in the whole imaged region. (D and E) SM tracklet density in center and periphery regions for WT (D) and mutant (E) CD36. In C–E, circles, red lines, shaded bars, and black asterisks (or lack thereof) are as in A and B. See Table 5 for number of cells, number of experimental repeats, and number of SM tracklets and speckles per ROI used for analysis.