Panel A shows the colocalization extent in the center region for CD36 wild type (CD36WT) with beta 1-integrin. The y-axis represents the colocalization extent, and the x-axis categorizes the data into overall, CD36 with CD9, and beta 1-integrin with CD9. Panel B displays similar data for the periphery region of CD36WT. Panel C presents the colocalization extent in the center region for CD36 with a G12V mutation (CD36G12V), and Panel D shows the periphery region for CD36G12V. Each boxplot includes individual cell measurements as circles, with the median, 25th and 75th percentiles, and 95 percent confidence interval of the median indicated. Inliers are marked with circles matching the box color, while outliers are in gray. The plots compare observed colocalization measures (blue) with those obtained when target molecules are replaced with points on a grid (nullTR, black) and when condition molecule locations are randomized (randC, green). Significant colocalization is marked with an asterisk, and significant effects of the condition molecule on colocalization are marked with a hash (#).
Analysis of CD36 colocalization with β 1 -integrin, conditional on CD9. (A–D) Details of the colocalization analysis of the indicated three molecules underlying the P values presented in Table S1, for target = CD36WT (A and B) or CD36G12V (C and D), reference = β1-integrin, and condition = CD9, in the center region (A and C) or the periphery region (B and D). The measure p(TwR) quantifies the extent of CD36 colocalization with β1-integrin regardless of either’s proximity or not to CD9 (“overall”). The measure p(TwR|TwC) quantifies the extent of CD36 colocalization with β1-integrin for the subset of CD36 colocalized with CD9 (“CD36 w/ CD9”). The measure prs(Tw(RwC)) quantifies the extent of CD36 colocalization with β1-integrin for the subset of β1-integrin colocalized with CD9 (“β1-integrin w/ CD9”). To assess the significance of colocalization for any of these sets/subsets, the observed colocalization measure (blue) is compared with that obtained when the target molecules (CD36) are replaced with points on a grid (“nullTR” [black]). To assess the significance of the effect of the condition, the observed p(TwR|TwC) and prs(Tw(RwC)) (blue) are compared with their counterparts when the condition molecule locations are randomized (“randC” [green]). To complete their significance testing, p(TwR|TwC) and prs(Tw(RwC)) are also compared with their corresponding p(TwR). Pairwise colocalization is taken as significant (indicated by *) when p(TwR) is significantly greater than its coincidental counterpart (nullTR) (P value ≤ 0.05). The effect of the condition molecule on colocalization is taken as significant (indicated by #) when p(TwR|TwC) or prs(Tw(RwC)) is significantly greater than its coincidental counterparts (both nullTR and randC) and its corresponding p(TwR) (combined type I error = 0.05 for the three tests). See Vega-Lugo et al. (2022) for more analysis details. All measures are shown as boxplots. For each box, individual circles indicate individual cell measurements, the central mark is the median, and the edges are the 25th and 75th percentiles. The notch around the median indicates the 95% confidence interval of the median. Circles with the same color as the box are inliers, while gray circles are outliers. See Table 4 for the number of cells, the number of experimental repeats, and the number of objects per channel per ROI used for analysis.