Panel A shows a boxplot comparing the density of CD36 detections per square micrometer in HDMECs and TIME cells transfected with Halo-CD36. The y-axis represents density (detections per square micrometer), and the x-axis shows the cell types. Individual cell data points are marked with circles, the mean of the group is indicated by a red line, and the standard deviation is shown as a shaded bar. Panel B includes a fixed-cell image of actin stained with phalloidin and a boxplot quantifying the average intensity of phalloidin in the center and periphery regions of the cell. The y-axis represents phalloidin intensity in arbitrary units, and the x-axis shows the regions. Panel C presents a fixed-cell image of CD36 and a boxplot quantifying the density of CD36 detections in the whole cell, center, and periphery regions. The y-axis represents density (detections per square micrometer), and the x-axis shows the regions. Panel D includes a fixed-cell image of beta-1-integrin and a boxplot quantifying the density of beta-1-integrin detections in the whole cell, center, and periphery regions. The y-axis represents density (detections per square micrometer), and the x-axis shows the regions. Panel E presents a fixed-cell image of CD9 and a boxplot quantifying the density of CD9 detections in the whole cell, center, and periphery regions. The y-axis represents density (detections per square micrometer), and the x-axis shows the regions. Panel F includes a fixed-cell image of CD81 and a boxplot quantifying the density of CD81 detections in the whole cell, center, and periphery regions. The y-axis represents density (detections per square micrometer), and the x-axis shows the regions. Panel G presents a fixed-cell image of CD151 and a boxplot quantifying the density of CD151 detections in the whole cell, center, and periphery regions. The y-axis represents density (detections per square micrometer), and the x-axis shows the regions. Panel H includes a fixed-cell image of CD36 with a specific mutation and a boxplot quantifying the density of CD36 detections in the whole cell, center, and periphery regions. The y-axis represents density (detections per square micrometer), and the x-axis shows the regions. In all images, solid red lines show the segmented cell edge, and dashed lines specify the boundary between the periphery and center regions. Scale bars in the images represent 5 micrometers.
CD36 expression level; density distributions of the various imaged molecules and actin in TIME cells. (A) Quantification of CD36 detection density in HDMECs (primary MVECs) endogenously expressing CD36 (n = 11 cells) and in TIME cells transiently transfected with Halo-CD36WT (n = 10 cells), in both cases as detected by the FA6-152 antibody against CD36. (B) Representative fixed-cell image of actin (stained using phalloidin) and quantification of its average intensity in the center and periphery regions separately. (C–H) Representative fixed-cell images of the indicated surface molecules and quantification of their detection densities in the whole imaged region, as well as in the center and periphery regions separately. In all images, solid red lines show the segmented cell edge; dashed lines specify the boundary between the periphery and center regions. Scale bars, 5 μm. The images shown in C, D, E, and H are, respectively, the same as the images shown in Fig. 1, B, C, D, and I. In all plots, circles, red lines, shaded bars, and asterisks are as in Fig 2. See Table 4 for number of cells, number of experimental repeats, and number of objects per channel per ROI used for analysis, except for A, where dataset size is indicated in the figure legend.