Panel A: A Northern blot analysis showing the expression of miR-BART3-5p (21 nucleotides), miR-BART5 (24 nucleotides), and 5S rRNA (120 nucleotides) in different cell lines. The cell lines include HK1, EBV-HK1, C666-1, 293, 293/pmiR-BART5, AGS/BX1, and AGS. The long exposure image is shown for miR-BART3-5p. Panel B: A Northern blot analysis showing the expression of pre-miR-BART5 (89 nucleotides), miR-BART5 (24 nucleotides), and U6 (106 nucleotides) in various cell lines. The cell lines include C666-1, NP01, NP02, NP03, NPC01, NPC02, NPC03, NPC04, NPC05, NPC06, NPC07, NPC08, and NPC09.
Expression of miR-BART5 in EBV-infected epithelial cells. (A) Northern blot analysis of EBV miRNAs in NPC and EBV-GC cell lines. HK1, HK1/EBV, and C666-1 are NPC cell lines (lanes 1–3). AGS/BX1 is an EBV-GC cell line (lane 6). Untransfected HEK293 cells (293; lane 4), HEK293 cells stably expressing miR-BART5 (293/pmiR-BART5; lane 5), and AGS cells (lane 7) were included as controls. Expression signal of miR-BART5 in HK1/EBV cells was visible only after long exposure (inset). The 5S rRNA served as a loading control. Although lanes 1–5 in the middle were from the same exposure of one experiment, lanes 6 and 7 were from another experiment. (B) Northern blot analysis of miR-BART5 in human NPC tissue samples. Three normal nasopharyngeal biopsies (NP01, NP02, and NP03; lanes 2–4) and nine NPC tumor samples (NPC01 to NPC09; lanes 5–8 and 11–15) were analyzed. C666-1 and latency III B95-8 lymphoblastoid cells (lanes 1, 9, and 10) were included as positive and negative controls, respectively. U6 small nuclear RNA served as a loading control.