FIP200 regulates mitophagy in B cells upon activation. (A) Schematic breeding strategy to generate MitoQC-B-Fip200^−/− mice and their littermate controls. (B) Dynamic of the MitoQC-GFP expression level in WT and MitoQC-B-Fip200^−/− B cells on day 3 of IL4+LPS stimulation. Data are representative of at least three independent experiments (n = 2–3 mice per treatment). One representative experiment is shown. (C) Representative cells from one WT mouse to demonstrate BDS scoring approach in gate R4 (GFP⁺mCherry⁺). (D) Representative plots of BDS scores of total live cells and CD138⁺ plasma cells measured by ImageStream; data were analyzed by the Kolmogorov–Smirnov test, ****P < 0.0001. Cells were cultured independently from two WT mice, with summary data from one mouse-derived culture shown. (E) Representative plots of BDS scores of WT and MitoQC-B-Fip200^−/− B cells on day 3 of IL4+LPS stimulation; data were analyzed by the Kolmogorov–Smirnov test, ****P < 0.0001. The experiment was performed at least three times (n = 1–3); representative data from cultures derived from one WT and one knockout mouse are shown here.