Removal of AMPK activation abolishes its action. (A and B) Top: Representative traces of current density recorded from HEK293F cells. Data were obtained from day-matched cells in control conditions or after treatment. (A) Bottom: Mean fully activated I/V curves normalized to capacitance measured under control conditions (black) and after 4-h incubation with either AICAR 1 mM (red) or Compound C 30 µM (blue); normalized conductance values from linear fitting were 1.09, 0.49, and 1.21 nS/pF, respectively. (B) Bottom: Similar set of measurements made under control conditions (black), and after 4-h incubation with either AICAR 1 mM (red) or the combination AICAR 1 mM + Compound C 30 µM (blue); normalized conductance values were 0.98, 0.53, and 0.99 nS/pF, respectively. See Tables 1 and S2 for statistical data analysis.