Panel A shows a heatmap of differentially expressed (DE) genes. The heatmap uses a color scale ranging from blue (low RNA expression) to red (high RNA expression), with EV, EV plus IL2, 1G12t, and 3A10t labeled along the x-axis. Panel B presents a heatmap of DE genes between 3A10t and EV across all samples, using the same color scale and labels. Panel C displays a heatmap of DE genes between 1G12t and EV across all samples, again using the same color scale and labels. Panel D features a Gene Ontology (GO) pathway analysis illustrating the top 5 featured pathways among genes significantly downregulated in EV plus IL-2 regulatory T cells (Tregs) compared to EV Tregs. The x-axis represents the gene ratio, and the y-axis lists the pathways. Circle size indicates the gene count of DE genes in each pathway, and color represents adjusted p-values. Panel E shows a Gene Ontology (GO) pathway analysis comparing the top 5 featured pathways among genes significantly downregulated in 3A10t samples compared to EV samples. The x-axis represents the gene ratio, and the y-axis lists the pathways. Circle size indicates the gene count of DE genes in each pathway, and color represents adjusted p-values.
Distinct transcriptomic program in Tregs expressing various tethered orthoIL-2 constructs (related to Fig. 5). (A) Heatmap of the expression of DE genes identified between EV+IL-2 and EV by all samples are shown. (B) Heatmap of the expression of DE genes identified between 3A10t and EV by all samples are shown. (C) Heatmap of the expression of DE genes identified between 1G12 and EV by all samples are shown. (D) Gene Ontology (GO) pathway analysis showcasing the top 5 featured pathways among genes significantly downregulated in EV+IL-2 Treg cells when compared with EV Tregs. (E) Gene Ontology (GO) pathway comparison analysis showcasing the top five featured pathways among genes significantly downregulated in 3A10t samples when compared with EV samples. In D and E, the plots depict the gene ratio of DE genes in each pathway, with the circle size indicating the gene count of DE genes in each pathway and the color representing the adjusted P values.