Panel A shows a scatter plot of Principal Component Analysis (PCA) comparing RNA-seq data of gamma delta T cells from P1 and healthy controls (HCs). The x-axis represents PC1 (42.46 percent) and the y-axis represents PC2 (10.79 percent). Different symbols and colors indicate ex vivo, overnight-rested, and IL-2 treated samples. Panel B displays a heatmap of differentially expressed genes related to the IL2-STAT5 signaling pathway. The x-axis lists gene names, and the y-axis shows sample groups (P1 and healthy controls). Panel C presents a heatmap of transcription factors and T cell maturation genes, with similar axes as Panel B. Panel D shows a heatmap of Th1/IFN-related genes. Panel E illustrates a heatmap of Th2-related genes. Panel F depicts a heatmap of Th17-related genes. Each heatmap uses a color scale to represent gene expression levels, with red indicating higher expression and blue indicating lower expression.
Differential gene expression in TCR γδ cells from P1 vs. HCs. (A) PCA of RNA-seq data comparing P1 with HC TCR γδ cells. The four clusters identified correspond to TCR γδ cells from P1 (red) and HCs (blue) in overnight-rested (dots) or ex vivo (triangles) samples. Cells treated with IL-2 for 30 min from HC (blue dots) and P1 (green dots) clusters were compared with IL-2–untreated cells. Samples for RNA-seq were collected from five HCs and from P1 at five time points; RNA-seq was performed in a single experiment. (B–F) Heatmaps of differentially expressed genes related to (B) the IL-2-STAT5 signaling pathway, (C) transcription factors and TCR γδ cell maturation, (D) Th1/IFN-γ, (E) Th2, and (F) Th17 profile. TCR γδ cells from P1 were isolated at five different time points. PCA, principal component analysis.