Panel A shows two flow cytometry plots. The left plot displays TCR V delta 2 versus TCR V delta 1, with axes labeled TCR V delta 2 and TCR V delta 1. The right plot shows CD3 versus TCR gamma delta gated on TCR V delta2/V delta 1 double negative cells, with axes labeled CD3 and TCR gamma delta. Panel B is a scatter plot summarizing TCRs expressed on PBMC-derived T cells from two healthy controls (black circles) and P1 (red circles), with axes labeled TCR percentage of CD3 positive cells and different TCR types. Panel C presents a flow cytometry plot of CD4 and CD8 expression gated on TCR V delta 2 cells from P1, with axes labeled CD8 and CD4. Panel D is a scatter plot summarizing CD4 and CD8 expression on PBMC-derived TCR V delta 2 cells from two healthy controls (black circles) and P1 (red circles), with axes labeled CD4/CD8 percentage within TCR V delta 2 cells and different subsets. Panel E shows a flow cytometry plot of CCR7 and CD45RA expression on TCR V delta 2 cells from P1, with axes labeled CCR7 and CD45RA. Panel F is a scatter plot summarizing CCR7 and CD45RA expression on PBMC-derived TCR V delta 2 cells from two healthy controls (black circles) and P1 (red circles), with axes labeled percentage within V delta 2 positive cells and different T cell types. Panel G displays two t-SNE plots of TCR V delta 2 cells from two healthy controls and P1, distributed in clusters 1 to 11. Panel H is a heatmap showing the clustering characterization of TCR V delta 2 cells from two healthy controls and P1 according to the expression of various markers.
TCR γδ cells of P1 predominantly express the Vδ2 chain. (A) Representative flow cytometry plot of TCR Vδ2 vs. TCR Vδ1 (left plot) on total T cells derived from PBMCs of P1 and CD3 vs. TCR γδ (right plot) on TCR Vδ2–/Vδ1– cells. (B) Summary of TCRs expressed on PBMC-derived T cells from two HCs (black circles) and P1 (red circles). (C) Representative flow cytometry plots of CD4 and CD8 expression on TCR Vδ2 cells derived from P1 PBMCs. (D) Summary of CD4 and CD8 expression on PBMC-derived TCR Vδ2 cells from two HCs (black circles) and P1 (red circles). DN: CD4−CD8– double negative. (E) Representative flow cytometry plots of CCR7 and CD45RA expression on TCR Vδ2 cells derived from P1 PBMCs. (F) Summary of CCR7 and CD45RA expression on PBMC-derived TCR Vδ2 cells from two HCs (black circles) and P1 (red circles). T naive: naive T cell; TCM: central memory T cell; TEM: effector memory T cell; TEMRA: effector memory T cell CD45RA-positive. (G) t-SNE of TCR Vδ2 cells from two HCs and P1 distributed in clusters 1–11 according to CD95, CD69, CD28, CD26, CD56, KLRG1, CD57, PD-1, NKp80, and TIGIT expression. (H) Heatmap of clustering characterization of TCR Vδ2 cells from two HCs and P1 according to the expression of the CD95, CD69, CD28, CD26, CD56, KLRG1, CD57, PD-1, NKp80, and TIGIT.