Figure S3.
Panel A shows a series of scatter plots for bone marrow gating strategy. The first plot displays forward scatter area (FSC-A) versus side scatter area (SSC-A) with a gated region for lymphocytes. Subsequent plots show gating for single cells, live cells, and various B cell populations based on markers like B220, IgM, CD43, CD19, and CD93. Panel B presents scatter plots for lymph node gating strategy for B cells and CD4 T cells. The first plot shows FSC-A versus SSC-A with a gated region for lymphocytes, followed by gating for single cells, live cells, and specific cell populations using markers like B220, CD4, and TCRb. Panel C shows scatter plots for T follicular helper cells, gated based on markers like CD4, CXCR5, and PD1. Panel D illustrates scatter plots for spleen gating strategy, showing gating for T1, T2, T3, follicular, and marginal zone compartments using markers like B220, CD23, and CD21. Panel E presents scatter plots for germinal centre B cells, comparing unimmunised control and immunised wildtype and Sorl1 minus slash minus mice, using markers like GL7 and CD38. Panel F shows scatter plots for plasma cell gating strategy, using markers like B220 and CD138.
B cell immunophenotyping gating strategies. (A) Bone marrow gating strategy for early B cell development and recirculating B cells. (B and C) Lymph node gating strategy for B cells, CD4 T cells, and T follicular helper cells. (D) Spleen gating strategy for T1, T2, T3, follicular, and marginal zone compartments. (E) Gating strategy for GC B cells, showing unimmunized control and immunized WT and Sorl1−/− mice. (F) Gating strategy for plasma cells. FMO indicates fluorescence minus one control.