Panel A: Four rows of microscopy images showing spermatids. Each row contains three images: a merged image, an image highlighting Ana1 in green, and an image highlighting Sas6 in red. The asterisks indicate the PCL structure. Scale bar is 2 micrometers. Panel B: Bar graph quantifying Sas6 localization within the Ana1-positive PCL structure. The y-axis represents the percentage of PCL, and the x-axis lists different genotypes. The bars show the percentage of Sas6-positively stained PCL, with values indicated above each bar. Panel C: Four rows of microscopy images showing spermatids at the leaf-shaped nuclei stage. Each row contains three images: a merged image, an image highlighting Ana1 in green, and an image highlighting Cep135 in red. The asterisks indicate the PCL structure. Scale bar is 2 micrometers. Panel D: Bar graph quantifying Cep135 localization within the Ana1-positive PCL structure. The y-axis represents the percentage of PCL, and the x-axis lists different genotypes. The bars show the percentage of Cep135-positively stained PCL, with values indicated above each bar.
Ana1-Cep135 interaction is required for the recruitment of Cep135 to the PCL structure. (A) Representative images of spermatids at the leaf-shaped nucleus stage in indicated genotypes. Colocalization of EGFP-Ana1 (green) with RFP-Sas6 (red) at the PCL structure. Scale bar, 2 µm. (B) Quantification of Sas6 localization within the Ana1-positive PCL structure. The percentage of the Sas6-positively stained PCL structure is indicated above each column. Data are from N = 80 per genotype collected from five independent testes (n = 10–15 PCL structures per testis). (C) Representative images of spermatids at the leaf-shaped nucleus stage in indicated genotypes. Colocalization of EGFP-Ana1 (green) with Cep135-mRuby3 (red) at the PCL structure. Scale bar, 2 µm. (D) Quantification of Cep135 localization within the Ana1-positive PCL structure. The percentage of Cep135-positively stained PCL structure is indicated above each column. Data are from N = 80 per genotype collected from five independent testes (n = 10–15 PCL structures per testis).