Figure S1.
Microscopy images stained with HPA antibody, GM130, and merged images for control siRNA treatment. Microscopy images stained with HPA antibody, GM130, and merged images for siRNA knockdown treatment. Each row is labeled with the name of the protein being studied. The merged images combine the HPA antibody staining in green, GM130 staining in white, and nuclear staining in blue. The rightmost column contains bar graphs quantifying the intensity measurements in the Golgi and nuclear compartments for each protein under control siRNA and siRNA knockdown conditions. The bar graphs have axes labeled with intensity units and include error bars representing variability.
Validation of DDR protein antibody specificity and localization, related to Fig. 1. Representative images of HeLa-K cells stained with antibodies against DDR proteins and the Golgi marker, GM130. Cells were treated with a control or an siRNA against the protein of interest to validate the specificity of the antibody. The changes in intensity measurements in the Golgi and nuclear compartment were quantified (n = 2 for all proteins except for POLQ, which had n = 1, biologically independent experiments with at least 200 cells analyzed for each treatment). Scale bar, 10 μm. Continued on Fig. S2 A.