Panel a shows a vertical bar graph. The x-axis lists the genes, grouped by BAR domain structure, and the y-axis represents the nTPM values. Panel b presents a heatmap. The x-axis lists the cell types, and the y-axis lists the genes. The color scale indicates relative expression levels, with yellow representing high expression and purple representing low expression.
BAR protein expression in endothelial cells. (a) Number of normalized transcripts per million (nTPM) in endothelial cells for all known genes encoding human BAR domain proteins recorded in the RNA single cell type dataset from the Human Protein Atlas (summarized expression levels per gene in 81 cell types from 31 datasets). Genes are ordered based on the structure of the BAR domain, as indicated below the graph. GAPDH is displayed as a comparison. (b) Hierarchically clustered expression levels of genes encoding human BAR domain proteins across arterial, venous, capillary, and lymphatic endothelial cell types. Human vasculature scRNA-seq data were obtained from the publicly available Tabula Sapiens dataset (Jones et al., 2022). Log10-transformed nCPM values per gene are indicated per gene, and a row-wise scaling was applied for color mapping across the cell types. The lower panel indicates gene expression of cluster markers for arterial (GJA5), venous (ACKR1), capillary (CA4), and lymphatic (LYVE1) endothelial cells.