Figure S2.
The illustration shows F-BAR domain proteins with VE-cadherin and GFP-tagged proteins showing varied junctional localization (white arrows). The BAR domain shows ARFIP1-GFP, ARFIP2-GFP, and PICK1-GFP localization relative to VE-cadherin. The BAR-PX domain shows SNX2-GFP, SNX6-GFP, SNX7-GFP, SNX8-GFP, and GFP-SNX9 localization patterns. The N-BAR domain shows BIN, Endophilin, SH3BP1, Amphiphysin1, and ARHGAP proteins with junctional enrichment (white arrows). The I-BAR domain shows BAIAP, BAIAP2L1, BAIAP2L2, MTSS1, and MTSS1L GFP-tagged protein localization. The BAR-PH domain shows APPL1-GFP, APPL2-GFP, ACAP1-GFP, ACAP2-GFP, ACAP3-GFP, ASAP1-GFP, ASAP2-GFP, and ASAP3-GFP showing intracellular and junctional localization.
Localization of BAR-GFP proteins in endothelial cells. Representative confocal microscopy images of transfected HUVECs expressing GFP-tagged BAR domain–containing protein (green) immunostained for VE-cadherin (magenta). White arrows highlight asymmetric AJs that are positive for the indicated BAR protein. Scale bar = 12 μm.