Figure 2.
Panel a: A schematic diagram showing the interaction between leader and follower cells during movement. The leader cell is on the right, and the follower cell is on the left. BAR protein is located at the adherens junction (AAJ) between the two cells. Panel b: Confocal microscopy images of human umbilical vein endothelial cells (HUVECs) expressing GFP-tagged BAR proteins (green) and immunostained for VE-cadherin (magenta). The images show regions of interest (ROI) at asymmetric adherens junctions (AAJs) with magnifications and single channels. Panel c: Confocal microscopy images of HUVECs immunostained for endogenous BAR proteins (green) and VE-cadherin (magenta). The images highlight regions of interest (ROI) with magnifications and single channels, indicating junctional recruited BAR proteins with orange arrowheads.
Multiple BAR proteins are recruited to AAJs. (a) Schematic representation of the AAJs between migrating leader and follower endothelial cells. (b) Representative confocal images from three independent experiments of HUVECs expressing GFP-tagged BAR proteins (green) immunostained for VE-cadherin (magenta). White squares indicate ROIs at AAJs, for which magnifications and single channels are shown below. (c) Representative confocal images of HUVECs immunostained for indicated endogenous BAR proteins (green) and VE-cadherin (magenta). White squares indicate ROIs, for which magnifications and single channels are shown below. Orange arrowheads indicate junctional recruited BAR proteins. Scale bars = 12, 10 and 5 μm.