Figure S1.
Panel a shows SIM microscopy images of a merged view and individual channels labeled Mitotracker, VE-cadherin-mScarlet, and PACSIN2-GFP. A dashed box marks the region of interest (ROI), which is enlarged in the lower row. Scale bars indicate 5 micrometers in the upper merged image and 3 micrometers in the ROI images. Panel b shows electron microscopy images of AAJ 2, including an x,y view, a zoomed-in x,y view of the boxed region, and corresponding y,z and x,z views. Dashed lines indicate section planes, arrows highlight membrane features, and scale bars indicate 0.5 micrometers and 0.3 micrometers. Panel c shows curvature quantification maps for AAJ 1, AAJ 2, and AAJ 3, with a color scale labeled ln(k) and corresponding values in nanometers, illustrating curvature distribution along the outlined membrane structures.
Cryo-SIM, FIB-SEM, and curvatures of endothelial AAJs. (a) Aligned SIM images of BOECs labeled for MitoTracker (cyan) and overexpressing VE-cadherin–mScarlet (magenta) and PACSIN2-GFP (green). ROI indicates region containing AAJs 1–3. (b) FIB-SEM orthoslices of AAJ2 to indicate a vesicle-like structure that extends with a single membrane from the double junctional membrane (black arrow). (c) Heatmaps of log-transformed subjunctional curvature values (k) with radius of curvature (nm) within AAJs. ln(k) = −1 to −5, radius = ∼10–600 nm. Scale bars = 5, 3, 0.5, and 0.3 μm.