Figure S4.
Panel A shows two sets of microscopy images side-by-side. The left set displays acetylated tubulin in HeLa cells treated with DMSO and tubacin. The right set displays total tubulin in HeLa cells treated with DMSO and tubacin. Cell contours are outlined with white curves. Panel B is a superplot showing the ratio of acetylated to total tubulin fluorescence intensity following different treatments. The x-axis is labeled with treatment types (DMSO and Tubacin), and the y-axis is labeled with the ratio of acetylated to total tubulin fluorescence intensity. The plot includes data points from three replicates with ten cells per replicate. Panel C is a flowchart illustrating the experimental pipeline for tubacin-induced stimulation of the secretory pathway. The flowchart includes steps such as pre-treatment, cargo accumulation at TGN, and cargo release, with specific time points and conditions indicated.
MT hyperacetylation upon tubacin treatment. (A) Representative confocal microscopy images of fixed HeLa cells stained for acetylated or total tubulin subjected or not to a treatment with 10 µM tubacin. Cell contours are indicated by white curves. Scale bar, 10 µm. (B) SuperPlots showing the ratio between fluorescence intensity of acetylated vs. total tubulin following the different treatments (n = 3 replicates; N∼10 cells per replicate). A two-sided parametric ratio paired t test was used. The P value is indicated in the plot. (C) Schematics of the pipeline followed in the tubacin-induced stimulation of the secretory pathway.