Figure S3.
Panel A shows fluorescence microscopy images of phosphorylated Myosin Light Chain Serine 19, F-actin, and merged images in cells cultured on Fibronectin for 1 hour, 4 hours, and 24 hours, and on Poly-L-lysine for 1 hour, 4 hours, and 24 hours. Panel B shows a superplot of phosphorylated Myosin Light Chain signal at the basal cell-substrate interface (arbitrary units), with the x-axis labeled Fibronectin and Poly-L-lysine at 1 hour, 4 hours, and 24 hours, and the y-axis labeled phosphorylated Myosin Light Chain signal at the basal cell-substrate interface (arbitrary units).
Early cell spreading on FN transiently increases pMLC levels. (A) Representative confocal microscopy images of HeLa cells seeded on FN or PLL for the indicated times, fixed, and stained for pMLC (pMLC Ser19-specific antibody) and F-actin (using Phalloidin-Alexa Fluor 647). Scale bars, 10 µm. (B) SuperPlot of the quantification of pMLC signal at the basal cell–substrate interface from cells treated as in A. A repeated-measures two-way ANOVA test was performed. P values using Tukey’s post hoc multiple comparison test are reported (N∼10 cells per biological replicate; n = 3 biological replicates).