Figure. S5.
Panel A shows immunofluorescence images of neurons stained for CHC using Antibody-1, Synaptophysin, and their merged images. The dot plot on the right displays Pearson correlation coefficients between CHC and Synaptophysin for Control, CAST/ELKS-KD, and EndA1-KD groups. Panel B shows similar immunofluorescence images using CHC Antibody-#2, with a corresponding dot plot of Pearson correlation coefficients. Panel C presents immunofluorescence images for AP-2 mu, Synaptophysin, and their merged images, along with a dot plot of Pearson correlation coefficients between AP-2 mu and Synaptophysin for the same groups. The dot plots indicate the correlation coefficients with statistical significance marked by asterisks.
Localization of CHC and AP-2 in CAST/ELKS- or EndA1-KD neurons. (A–C) Rat hippocampal neurons in culture (control, CAST/ELKS-KD, or EndA1-KD) were immunostained for CHC (A and B; anti-CHC antibodies: A, CHC antibody-#1 [catalog no. ab21679; Abcam]; B, CHC antibody-#2 [catalog no. 2410; Cell Signaling]), AP-2 μ (C), and synaptophysin. Dot plots on the right show correlation coefficients between CHC and synaptophysin (A: n = 10 images per group; B: control, n = 19; CAST/ELKS-KD, n = 18; EndA1-KD, n = 24), as well as between AP-2 and synaptophysin (C: control, n = 19; CAST/ELKS-KD, n = 20; EndA1-KD, n = 17). P values were calculated relative to control neurons using the nonparametric Mann–Whitney test, **P < 0.01. Scale bars, 2 µm.