Panel A shows two grayscale microscopy images. The top image shows membrane marker labeling of an embryo from a germ cell-less heterozygous mother, and the bottom image shows an embryo from a germ cell-less homozygous mutant mother. The scale bar is 20 micrometers. Panel B is a diagram illustrating the measurement of pole bud length and width, with red dashed lines indicating the dimensions. Panel C is a scatter plot showing the width of pole buds measured in micrometers on the y-axis. The x-axis labels refer to embryo counts from germ cell-less heterozygous and germ cell-less homozygous mutant mothers, with data points representing individual measurements and bars indicating the mean and standard deviation. The label ns indicates no significant difference. Panel D is a scatter plot showing the length of pole buds measured in micrometers on the y-axis. The x-axis labels refer to embryos from germ cell-less heterozygous and germ cell-less homozygous mutant mothers, with data points representing individual measurements and bars indicating the mean and standard deviation. The label less than 0.0001 indicates a statistical significant difference.
Pole bud membrane shape in embryos from gcl−/− mothers is flattened due to an imbalance in phosphoinositides. (A) Nuclear cycle 10 embryos from gcl+/− and gcl−/− mothers also expressing the membrane marker tdKatushka2:CAAX were live imaged on their side. These pole buds are tightly clustered together but have not cellularized. Scale bar = 20 µm. (B) Diagram of pole bud length and width measurements. (C) The width of pole buds was measured and plotted. Bars represent the mean ± standard deviation. (n = 10 buds from embryos derived from gcl+/− mothers, n = 13 buds from embryos derived from gcl−/− mothers, Mann–Whitney test). (D) The length of pole buds was measured and plotted. Bars represent the mean ± standard deviation. (n = 10 buds from embryos derived from gcl+/− mothers, n = 13 buds from embryos derived from gcl−/− mothers, Mann–Whitney test).