Panel A: This panel shows fluorescent images of embryos immunostained with anti-HA and anti-Vasa antibodies. The images depict maximum intensity projections spanning the area of PGC formation. The images are labeled as Control, p60-WT, and p60-delta SH2, with scale bars indicating 100 micrometers. Panel B: This panel presents a scatter plot showing the number of PGCs in nuclear cycle 13-14 embryos from mothers of indicated genotypes. The x-axis represents the maternal genotype, and the y-axis represents the number of PGCs per embryo. The plot includes statistical annotations indicating significant differences. Panel C: This panel shows fluorescent images of embryos from mothers overexpressing HA-tagged p60 with a germplasm-targeting 3UTR (TCEp3). The images depict maximum intensity projections spanning the area of PGC formation, with scale bars indicating 50 micrometers. Panel D: This panel presents a scatter plot showing the number of PGCs in nuclear cycle 13-14 embryos from mothers of indicated genotypes. The x-axis represents the maternal genotype, and the y-axis represents the number of PGCs per embryo. The plot includes statistical annotations indicating significant differences. Panel E: This panel shows a schematic diagram depicting p60-WT and variant constructs used in the experiment.
Decreasing PI3K activity rescues the gcl−/− phenotype and increases PGC formation. (A) Embryos from mothers overexpressing HA-tagged p60-WT and p60-ΔSH2 were immunostained with anti-HA and anti-Vasa to confirm expression and count PGCs. Images depict maximum intensity projections spanning the area of PGC formation. Scale bar = 100 µm. (B) PGCs in nuclear cycle 13–14 embryos from mothers of indicated genotype were counted and plotted from A. The maternal genotype of control embryos is maternal-tubulin-GAL4-VP16/+; maternal-tubulin-GAL4-VP16/+. Bars represent the mean ± standard deviation. (n > 20, Mann–Whitney test). (C) Embryos from mothers overexpressing HA-tagged p60 with a germplasm-targeting 3′UTR (TCEp3) were immunostained with anti-HA and anti-Vasa to confirm expression and count PGCs. Images depict maximum intensity projections spanning the area of PGC formation. Scale bar = 50 µm. (D) PGCs in nuclear cycle 13–14 embryos from mothers of the indicated genotype were counted and plotted from C. The maternal genotype of control embryos is maternal-tubulin-GAL4-VP16/+; maternal-tubulin-GAL4-VP16/+. Bars represent the mean ± standard deviation. (n > 20, Mann–Whitney test). (E) Schematic depicting p60-WT and variant constructs used.