Panel A: Cross-sectional microscopy images show maximum intensity projections of embryos immunostained for GFP and Vasa. The p60 protein, tagged with GFP, is uniformly distributed throughout the embryo. Vasa protein is concentrated at the posterior pole. The inset provides a close-up view of the posterior pole, highlighting the concentration of Vasa. Scale bars are 100 micrometers for the whole embryo and 50 micrometers for the inset. Panel B: Cross-sectional microscopy images show maximum intensity projections of embryos immunostained for GFP and Vasa. The p60 protein, tagged with GFP, localizes to furrows and is excluded from pole cells. Vasa protein remains concentrated at the posterior pole. The inset provides a close-up view of the posterior pole, highlighting the concentration of Vasa. Scale bars are 100 micrometers for the whole embryo and 50 micrometers for the inset.
(related to Fig. 5): p60, the regulatory subunit of PI3K in Drosophila, is uniformly distributed across the embryo prior to PGC formation, but by nuclear cycle 14 localizes to furrows and is excluded from pole cells. (A) Nuclear cycle 9–10 embryos shortly before pole cell formation from mothers expressing a p60-GFP fosmid were immunostained for anti-GFP and anti-Vasa. The inset shows a close-up of the posterior pole. Images depict maximum intensity projections spanning the area of PGC formation. Scale bar = 100 µm for whole embryo, 50 µm for inset (B) Nuclear cycle 13–14 embryos after pole cell formation from mothers expressing a p60-GFP fosmid were immunostained for anti-GFP and anti-Vasa. The inset shows a close-up of the posterior pole. Images depict maximum intensity projections spanning the area of PGC formation. Scale bar = 100 µm for whole embryo, 50 µm for inset.