Figure S1.
The cross-sectional views of embryos at nuclear cycle 13-14, showcasing the effects of RNA interference (RNAi) on different genes. Each row represents a different genotype: csw, dsor, and rolled. The columns show staining for Vasa (green), dpERK (magenta), and a merged image with DAPI (blue). The top row shows the csw genotype with Vasa staining. The middle row shows the dsor genotype with Vasa staining. The bottom row shows the rolled genotype with Vasa staining. The top row shows the csw knockdown with dpERK staining. The middle row shows the dsor knockdown with dpERK staining. The bottom row shows the rolled knowckdown with dpERK staining. The top row shows the csw knockdown with merged Vasa, dpERK, and DAPI staining. The middle row shows the dsor knockdown with merged Vasa, dpERK, and DAPI staining. The bottom row shows the rolled knockdown with merged Vasa, dpERK, and DAPI staining.
(related to Fig. 2): Knockdown of csw, dsor, and rolled fails to rescue PGC defect in embryos from gcl−/− mothers. Representative embryos of indicated maternal genotypes, nuclear cycle 13–14. Fixed embryos were immunostained with anti-Vasa (Green) and anti-dpERK (magenta). DNA (blue). Scale bar = 50 μm.