The diagram illustrates the process starting with PCM1 monomers self-associating to form a scaffold. This is followed by the formation of an early granule, which is dynamic. Progressive recruitment of client proteins occurs over time, leading to the maturation into larger, stable satellite assemblies. The internal organization of mature satellites is shown with a layered architecture consisting of an inner core, intermediate zone, core-shell interface, shell, and outer shell. Mature centriolar satellites localize around the centrosome and facilitate efficient ciliogenesis.
Model for hierarchical assembly of CS driven by PCM1 multimerization. The cartoon shows how the initial multimerization of PCM1 provides a scaffold for the ordered assembly of CS components, as described by Begar and colleagues (7). PCM1 monomers self-associate to initiate scaffold formation, followed by early granule formation (30′), progressive recruitment of client proteins (2–16 h), and maturation into larger, stable satellite assemblies. An internal organization of mature satellites is shown with a layered architecture consisting of an inner core (shown in red), intermediate zone (orange), core–shell interface (yellow), shell (light green), and outer shell (dark green). The components of the different layers do not all assemble at the same time. Mature CS localize around the centrosome and facilitate ciliogenesis.