Panel A is a scatter plot. The x-axis represents Force in piconewtons, and the y-axis represents the Branch renucleation ratio. Different colors and symbols represent varying concentrations of actin (0.4 micromolar and 0.2 micromolar). Panel B is a line graph showing the Survival fraction over time for different forces (3.16 piconewtons, 3.93 piconewtons, and 5.16 piconewtons) with actin concentration color-coded. The x-axis represents Time in seconds, and the y-axis represents Survival fraction. Panel C is another line graph showing the Survival fraction over time for different forces (3.27 piconewtons, 3.6 piconewtons, and 5.12 piconewtons) with actin concentration color-coded. The x-axis represents Time in seconds, and the y-axis represents Survival fraction. Panel D is a line graph showing the Survival fraction over time for different forces (2.08 piconewtons, 2.43 piconewtons, and 4.44 piconewtons) with actin concentration color-coded. The x-axis represents Time in seconds, and the y-axis represents Survival fraction. Each panel shows trends and patterns in the data, with varying survival fractions and renucleation ratios under different experimental conditions.
ADP-BeFx-Arp2/3 complex renucleates for several rounds with a high renucleation ratio. (A) Branch renucleation ratio as a function of force from Arp2/3 complex branches in the ADP-BeFx state. Renucleation is probed in the presence of indicated concentrations of actin, in the absence of BeFx in solution. (B–D) Branch survival fractions from the same initial population of Arp2/3 complexes attached to mother filaments for different forces and actin concentrations. Actin concentration color code is the same as in the top panel, the darkest tone is the first round of branches, the lightest the third round (number of branches per conditions: green, 0.4 µM actin: 60 [5.16 pN], 41 [3.16 pN], 29 [3.93 pN]; orange, 0.4 µM actin: 78 [5.12 pN], 50 [3.6 pN], 44 [3.27 pN]; blue, 0.2 µM actin: 60 [4.44 pN], 36 [2.43 pN], 9 [2.08 pN]).