Panel A shows a schematic diagram illustrating mixed bone marrow transplantation into lethally irradiated recipient mice. Panel B shows flow cytometry plots defining Foxp3-positive regulatory T-cell subpopulations, including suppressive, naïve, memory-like, and recently activated/T follicular regulatory-like cells. Panel C shows a paired scatter plot with x-axis Cell source and y-axis Percentage of Foxp3-positive cells among CD4 T cells. Panel D shows paired scatter plots with x-axis Cell source and y-axis Percentage of suppressive regulatory T cells, naïve regulatory T cells, memory-like regulatory T cells, or recently activated/T follicular regulatory-like regulatory T cells. Panel E shows a paired scatter plot with x-axis Cell source and y-axis Percentage of Foxp3-positive cells among CD4 T cells. Panel F shows paired scatter plots with x-axis Cell source and y-axis Percentage of suppressive regulatory T cells, naïve regulatory T cells, memory-like regulatory T cells, or recently activated/T follicular regulatory-like regulatory T cells.
Differentiation of islet Foxp3 + Tregs is intrinsically controlled by cell surface CD137. (A) Design of the MBMC experiment. (B) Gating strategy for naïve (CD62L+), suppressive (CD62L− CD103+ and/or CXCR6+), memory-like (CD62L−CD103−CXCR6−CD127+), and recently activated/Tfr-like (CD62L−CD103−CXCR6−CD127−) subpopulations of Foxp3+ Tregs in the islets of the Cre+-Tnfrsf9fl/fl and NOD.CD45.2 MBMCs. (C) Frequencies of Cre+-Tnfrsf9fl/fl and NOD.CD45.2-derived Foxp3+ Tregs among the respective CD4 T cells in the islets of the MBMCs. *P < 0.05 by a paired t test. Results are summarized from three experiments. (D) Frequencies of Cre+-Tnfrsf9fl/fl and NOD.CD45.2-derived suppressive, naïve, memory-like, and recently activated/Tfr-like subpopulations within the respective Foxp3+ Tregs in the islets of the MBMCs. *P < 0.05 by a paired t test. ns: not significant. Results are summarized from three experiments. (E) Frequencies of Cre+-Tnfrsf9+/+ and NOD.CD45.2-derived Foxp3+ Tregs among the respective CD4 T cells in the islets of the control MBMCs. (F) Frequencies of Cre+-Tnfrsf9+/+ and NOD.CD45.2-derived suppressive, naïve, memory-like, and recently activated/Tfr-like subpopulations within the respective Foxp3+ Tregs in the islets of the control MBMCs. ns: not significant by a paired t test. Results are summarized from three experiments.