Panel A shows bar graphs depicting the percentages of CD8 T cells, CD4 T cells, B cells, and myeloid cells. The y-axis represents the percentage of cells, and the x-axis compares WT and KO mice. Significant differences are marked with asterisks, indicating P less than 0.05, while non-significant differences are indicated where appropriate. Panel B presents a bar graph showing the percentage of Foxp3-positive regulatory T cells among CD4 T cells, with a significant difference noted. Panels C and D display bar graphs of the frequencies of CD44-high CD127-low, CD44-high CD127-high, and CD44-low CD8 T cells and Foxp3-negative CD4 T cells, respectively, with significant differences marked. Panels E and F show bar graphs of the frequencies of Ki67-positive cells in various subsets of CD8 and CD4 T cells, with significant differences indicated by asterisks. The data is summarized from 3 to 5 experiments.
CD137 deletion in Foxp3 + Tregs causes enhanced T cell activation in pancreatic islets. (A) Frequencies of T cells, B cells (CD19+), and myeloid cells in islets of 8- to 11-wk-old prediabetic Cre+-Tnfrsf9+/+ (WT) and Cre+-Tnfrsf9fl/fl (KO) females. *P < 0.05 by an unpaired t test. ns: not significant. (B) Frequency of Foxp3+ Tregs in islets of 8- to 11-wk-old prediabetic Cre+-Tnfrsf9+/+ (WT) and Cre+-Tnfrsf9fl/fl (KO) females. *P < 0.05 by an unpaired t test. (C and D) Frequencies of CD44highCD127low, CD44highCD127high, and CD44low CD8 T cells (C) and Foxp3− CD4 T cells (D) in islets of 8- to 11-wk-old prediabetic Cre+-Tnfrsf9+/+ (WT) and Cre+-Tnfrsf9fl/fl (KO) females. *P < 0.05 by an unpaired t test. ns: not significant. (E and F) Frequencies of Ki67+ cells in the indicated subsets of CD8 (E) and CD4 (F) T cells in islets of 8- to 11-wk-old prediabetic Cre+-Tnfrsf9+/+ (WT) and Cre+-Tnfrsf9fl/fl (KO) females. *P < 0.05, **P < 0.005; ***P < 0.0005 by an unpaired t test. ns: not significant. Results in A–F are summarized from three to five experiments.