Figure S2.
Panel A shows three separate microscopy images of cells. The first image displays cells with green fluorescence indicating the presence of su9-mTagBFP2-DHFR after 48 hours of incubation with MTX. The second image shows cells with purple fluorescence indicating the presence of mCherry-Fis1. The third image is a merged view of the first two images, showing colocalization of the green and purple fluorescence. Panel B shows DAPI staining of double positive cells. Panel C shows three sets of microscopy images. The first set displays cells with green fluorescence indicating import-competent mitochondria. The second set shows cells with purple fluorescence indicating import-defective mitochondria. The third set is a merged view of the first two sets, highlighting that transferred import-competent and import-defective mitochondria recapitulate the morphologies seen in HeLaGAL cell lines.
Fis1, DAPI, and astrocytes. (A) Representative image of su9-mTagBFP2-DHFR cell line following 48 h incubation with MTX (import-defective) and transfection with mCherry-Fis1, showing co-localization. (B) Representative images of double-positive cells from a 48 h co-culture of su9-EGFP line (import-competent) and su9-mScarlet-DHFR line in the presence of MTX (import-defective) following fixation and DAPI staining. (C) Examples of double-positive cells from a co-culture of primary astrocytes expressing either cox8a-DsRed (import-competent) or su9-EGFP-DHFR in the presence of MTX (import-defective). Scale bars 20 μm.