Figure S1.
Panel A shows a series of flow cytometry plots demonstrating gating strategy. The first plot displays side scatter area (SSC-A) versus forward scatter area (FSC-A) with a gated region labeled Cells. The second plot shows forward scatter height (FSC-H) versus forward scatter area (FSC-A) with a gated region labeled Singlets. The third plot presents APC-Cy7-A (Zombie NIR) versus forward scatter area (FSC-A) with a gated region labeled Alive. Panel B contains four flow cytometry plots for identifying BFP-positive/mScarlet-positive cells. Each plot shows BUV496-A (BFP) versus PE-A (mScarlet) with different cell conditions: Parental, su9-mTagBFP2 monoculture, su9-mScarlet-DHFR monoculture, and su9-mScarlet-DHFR plus su9-mTagBFP2 plus MTX. Quartiles are labeled within each plot. Panel C features two histograms showing double-positive cell count versus mScarlet or EGFP intensity for different cocultivation time points. The histograms are color-coded to represent different hours of coculture with MTX.
Cells subject to IMT can be isolated by FACS. (A) Gating strategy for isolation of cells that are singlets and alive. (B) Four-way gating system for identification of BFP+/mScarlet+ cells. (C) Representative histograms of double-positive cell count vs. mScarlet or EGFP intensity for each time point of co-cultivation time course. Q; quartile. SSC-A; side scatter area. FSC-A; forward scatter area. Comp; compensation.