Panel A shows bar graphs and microscopy images of kidney sections from WT and Sting minus/minus mice subjected to sham or UUO, stained with Masson's trichrome and PSR. The x-axis represents different groups, and the y-axis represents the fibrotic area percentage. Panel B displays similar data for WT and Myd88 minus/minus mice. Panel C presents similar data for WT and Tlr3 minus/minus mice. Panel D shows similar data for WT and Mda5 minus/minus mice. Panel E includes microscopy images and bar graphs of kidney sections from WT and Mavs minus/minus UUO mice, stained with Masson's trichrome, PSR, alpha-smooth muscle actin, and fibronectin. The x-axis represents different groups, and the y-axis represents the fibrotic area percentage or optical density. Panel F shows microscopy images and bar graphs of kidney sections from sham and UUO mice with or without RIG-I inhibitor (cFP) treatment, stained with Masson's trichrome, PSR, alpha-smooth muscle actin, and fibronectin. The x-axis represents different groups, and the y-axis represents the fibrotic area percentage or optical density. Each panel shows significant differences in fibrotic areas and staining intensities among the different groups.
Inhibition of STING, Myd88, TLR3, or RIG-I/MAVS signaling attenuates renal fibrosis in mice. (A–D) Representative images and quantitation of the fibrotic areas with Masson’s trichrome and PSR staining in the kidneys from WT, Sting–/– (A), Myd88−/− (B), Trl3−/− (C), and Mda5−/− (D) mice subjected to sham or UUO mice. Scale bars = 50 μm. (E) Representative images and quantitative analysis of Masson’s trichrome, PSR, α-SMA, and fibronectin staining in kidneys from WT and Mavs–/– UUO mice. Scale bar = 50 μm. (F) Representative images for Masson’s trichrome, PSR, α-SMA, and fibronectin staining in the kidneys of sham and UUO mice with or without cFP treatment. Scale bars = 50 μm. Data are pooled from two independent experiments with a total of five to six mice per group. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001, by two-way ANOVA with Tukey’s multiple-comparison test (A–E) or one-way ANOVA with Tukey’s multiple-comparison test (F).