Figure S4.
Panel A shows a series of microscopy images taken at different time points (0, 2, 5, 15, 30, and 60 minutes) to analyze the detection of AMY and the expression of the ZG membrane glycoprotein 2 (GP2) in CCK-8 treated AR42J cells. The images are divided into three columns: AMY (green), GP2 (red), and Merge (combination of AMY and GP2 with DAPI staining in blue). Each row represents a different time point. Scale bars are 2 micrometers. Panel B presents line scan profiles of fluorescence intensity for AMY (green curves) and GP2 (red curves) along a white line in the merged images from Panel A. The graphs show fluorescence intensity on the y-axis and distance in micrometers on the x-axis. The index of correlation (Icorr) is calculated to measure the amount of colocalization between AMY and GP2 stains, with values ranging from 0 to 1.
Time course of ZG activation in AR42J cells induced by CCK-8. (A) Time course analysis of AMY detection and of the ZG membrane GP2 expression in CCK-8–treated AR42J cells by double immunofluorescence microscopy using AMY- and GP2-specific antibodies (α-AMY and α-GP2, respectively). Scale bars, 2 µm. (B) Line-scan profiles (white bars in merged images on the right in A) of fluorescence intensity for the analysis of colocalization of AMY (green curves) and GP2 (red curves). The index of correlation (Icorr) was calculated to measure the amount of colocalization between the AMY and GPS stains in the images (values typically range from 0 to 1, where 0 indicates no colocalization, and 1 denotes complete colocalization).