Figure 4.
Panel A: Four bar graphs showing the transcription levels of GBP1, IFIT1, RSAD2, and USP18 genes in STAT2 deficient U6A fibrosarcoma cells transfected with various STAT2 variants and treated with IFN-alpha 2A. The horizontal axis labels include NT, EV, WT, K490Qfs 41, R510X, INSin9, INSin8, and DELex9. The vertical axis represents the ratio of gene expression in stimulated versus unstimulated cells as a percentage of wild type (WT). Each bar graph shows the mean and standard deviation of the gene expression levels. Panel B: Four bar graphs showing the transcription levels of the same genes in cells treated with IFN-gamma. The horizontal and vertical axes are labeled similarly to Panel A. Each bar graph shows the mean and standard deviation of the gene expression levels.
The STAT2 variants impair downstream type I IFN signaling. (A and B) Transcription levels of GBP1, IFIT1, RSAD2, and USP18 assessed by RT-qPCR on STAT2-deficient U6A fibrosarcoma cells transfected with empty vector (EV), WT STAT2, R510X (positive control), K490Qfs*41, INSin9, INSin8, and DELex9. Cells were pretreated with 10,000 IU/ml IFN-α2A (A) or 1,000 IU/ml IFN-γ (B) for 6 h. A representative blot from two experiments is shown.