Panel A shows a UMAP graph with various annotated testis cell clusters. Different colors represent different cell types, such as germinal proliferation, cyst cells, spermatocytes, and pigment cells. Panel B displays a UMAP graph indicating cid expression levels in adult testis, with black representing lower expression and red showing higher expression. Panel C presents a UMAP graph of cal1 expression in adult testis, highlighted in green. Panel D illustrates a UMAP graph of Cenp-C expression in adult testis, shown in blue. Panel E features a line graph depicting the average Log fold-change of cal1, Cenp-C, nanos, bam, Rbp4, and Protamine A across different stages of spermatogenesis, including 4CC plus 8CC, 16CC S1, 16CC S6, and 64CC.
Single-cell RNA-sequencing expression data for centromere proteins and known markers of spermatogenesis. (A) UMAP graph displaying annotated testis cell clusters from the Fly Cell Atlas (Li et al., 2022), visualized using the SCope platform. (B) UMAP graph indicating expression levels of cid in adult testis. Black represents cells with lower expression, and red shows higher expression. (C) UMAP graph displaying cal1 expression within the adult testis in green. (D)Cenp-C expression within the adult testis is shown in blue. (E) Graph depicting the average log fold change of cal1 and Cenp-C, along with several known markers of spermatogenesis, including nanos and bam (spermatogonia and spermatocytes), Rbp4 (spermatocytes), and Protamine A (spermatocytes and spermatids). 4CC + 8CC data were derived from the mid/late-proliferating spermatogonia cluster in the 10× dataset. Spermatocyte 1 and 6 clusters were mapped to early and late prophase I using known expression patterns of nanos, bam, and Rbp4. Early/mid-elongation stage spermatids were used as the 64CC dataset. UMAP, Uniform Manifold Approximation and Projection.