Figure 3.
Panel A shows electron micrographs comparing NM 2B filaments (left) and NM 2B–2C NHT filaments (right). Numerous elongated bipolar filaments are dispersed across each field, with thicker filament ends and thinner central regions. Panel B shows higher-magnification electron micrographs of individual filaments. Images compare NM 2B, NM 2B–2C NHT, NM 2B delta NHT with a reduced bare zone, and NM 2B delta NHT lacking a visible bare zone. Panel C shows electron micrographs comparing NM 2C filaments (left) and NM 2C–2B NHT filaments (right), displaying multiple bipolar filaments distributed throughout the field. Panel D presents higher-magnification views of individual filaments from NM 2C, NM 2C–2B NHT, NM 2C delta NHT with a reduced bare zone, and NM 2C delta NHT with no visible bare zone. Scale bars represent 100 nanometers.
NHTs of NM-IIB and NM-IIC control their filament size. (A) Electron micrographs of polymerized NM-IIB and NM-IIB-IIC-NHT. (B) Zoomed-in view of polymerized NM-IIB, NM-IIB-IIC-NHT, and NM-IIBΔNHT. (C) Electron micrographs of polymerized NM-IIC and NM-IIC-IIB-NHT. (D) Zoomed-in view of polymerized NM-IIC, NM-IIC-IIB-NHT, and NM-IICΔNHT. Myosins were polymerized under the same conditions as described in Fig. 1. Note: The image shown in Fig. 3 A (left panel) is identical to that shown in Fig. 1 C (top panel), and the image shown in Fig. 3 C (left panel) is identical to that shown in Fig. 1 D (top panel). These images are included in multiple figures to facilitate comparison of the relevant data.