Figure S5.
Panel A shows flow cytometry plots analyzing CD36, CD64, MARCO, MERTK, and TIM4 expression in CCR2-positive and CCR2-negative macrophage and monocyte populations from liver and blood samples of primary sclerosing cholangitis (PSC) and control (non-PSC) patients. Panel B shows a histogram of normalized hepatocyte uptake signature expression in Kupffer cells (KCs). The horizontal axis represents normalized expression values, and the vertical axis represents cell count distributions, with a marked cutoff threshold. Panel C presents a UMAP feature plot displaying normalized liver X receptor alpha (LXRa) expression across KCs, macrophages and monocytes. The color scale represents relative messenger RNA expression levels. Panel D shows a UMAP feature plot displaying normalized CCR2 expression across KCs, macrophages and monocytes. The color scale represents relative messenger RNA expression levels.
Blood-derived and hepatic Mφ characterization in a PSC patient and a non-PSC control. (A) FACS plots reporting gating strategy for analysis of CD36, CD64, MARCO, MERTK, and TIM4 in CD66b−CD68+CD14+CCR2− and CD66b−CD68+CD14+CCR2+ Mφ/monocytes isolated from the liver and the blood of a non-PSC patient (Control) and a PSC patient. Analysis of Kupffer cells (KCs), Mφ, and monocytes from scRNAseq data from Andrews et al. (2024) of healthy, PBC, and PSC livers. (B) Histogram of normalized expression of hepatocyte uptake signature comprising ALB, CYP3A4, and APOA1. Cutoff indicates the 90th percentile. (C and D) Normalized gene expression of (C) LXRa and (D) CCR2 on UMAP from Fig. 5 B.