Figure S2.
Panel A shows fluorescence microscopy images comparing capacity of Mdr2-/- and control bone marrow-derived or liver macrophages to engulf apoptotic thymocytes or apoptotic hepatocytes after 45- and 120-minute co-culture. Magenta labels macrophages, green labels apoptotic cells, and inset zooms highlight engulfed apoptotic material within macrophages. Panel B shows representative flow-cytometry plots and quantitative scatter plots measuring macrophage efferocytic efficiency after coculture with apoptotic thymocytes or hepatocytes.
Efferocytic capacity of bone marrow-derived and hepatic Mφ from Mdr2−/− mice. (A and B) (A) Representative images, and (B) FACS plots from liver Mφ and graphs reporting the frequency of Mdr2+/+ and Mdr2−/− Mφ differentiated from the bone marrow (BMDMs) or isolated from the liver (liver Mφ), engulfing in vitro apoptotic thymocytes (aT) or apoptotic hepatocytes (aH) upon 45 min or 120 min coculture. Efferocytic Mφ are identified as CD11b+F4/80+ cells, expressing the apoptotic cell tracker. Scale bar, 20 μm. n = 7–10; mean ± SEM, Mann–Whitney U test. *P < 0.05.