Panel A shows scatter plots quantifying resident and infiltrating hepatic macrophage frequencies across different disease progression stages. Panel B presents scatter plots comparing phagocytic receptor expression in infiltrating and resident hepatic macrophage populations in Mdr2-/- mice and controls (Mdr2+/+). Panel C shows representative flow-cytometry plots illustrating CD36, MERTK, TIM4, and CD64 expression in hepatic macrophages. Panel D presents t-SNE maps visualizing phagocytic receptor-expression patterns across resident and infiltrating macrophage subpopulations in control (Mdr2+/+) and diseased livers (Mdr2-/-).
Phagocitic profile of hepatic Mφ during cholangitis progression in Mdr2 −/− mice . (A) Graphs representing the frequencies of resident and infiltrating Mφ in Mdr2−/− and Mdr2+/+ mice at 8, 12, and 25 wk of age. Two independent experiments were performed. Each data point represents one independent sample. n = 9–11; mean ± SEM. Mann–Whitney U test. (B and C) (B) Pooled data and (C) representative FACS plot reporting frequency of resident and infiltrating Mφ expressing the phagocytic receptors CD36, MERTK, TIM4, and CD64 isolated from the liver of Mdr2+/+ (clear dots) and Mdr2−/− (filled dots) mice at 8 and 25 wk of age. n = 9–12 samples/time point. Mean ± SEM. Mann–Whitney U test. (D) t-SNE maps showing the expression of the phagocytic receptors CD36, MERTK, TIM4, and CD64 in infiltrating and resident Mφ within the Ly6C+ and Ly6C− clusters in 8- and 25-wk-old Mdr2+/+ and Mdr2−/− mice. **P < 0.01; ***P < 0.001; ****P < 0.0001.