Panel A shows a kymograph with three sections: Col1-mNG in green, Lysotracker in magenta, and a merged view. The traces of Col1-mNG and Lysotracker do not overlap. Panel B is a scatter plot displaying the Manders colocalization coefficient M2 for Col1A1-mNG and Lysotracker red in 21 cells from two independent experiments, indicating low colocalization. Panel C contains fluorescence microscopy images of fixed activated LX-2 cells stained with antibodies against Col1A1 in green and LC3 in magenta, with zoomed insets showing PC1 condensates not near autophagosomes. Panel D shows fluorescence images of fixed activated LX-2 cells stained with antibodies against Col1A1 in green and G3BP1 in magenta, indicating a homogenous G3BP1 signal.
PC1 condensates do not trigger a cellular stress response. (A) Kymograph corresponding to a line drawn through a cell in Video 5 showing no overlap between the Col1-mNG (green) and LysoTracker (magenta) traces. (B) Manders’ colocalization coefficient M2 for Col1A1-mNG and LysoTracker Red in 21 cells from two independent experiments showing low colocalization between PC1 condensates and lysosomes. (C) Fluorescence images of fixed activated LX-2 cells stained with antibodies against endogenous Col1A1 (green) and LC3 (magenta) (top), and zoomed insets (bottom) showing PC1 condensates are not in the vicinity of autophagosomes (scale bars: 10 µm; inset: 2 µm). (D) Fluorescence images of fixed activated LX-2 cells stained with antibodies against endogenous Col1A1 (green) and G3BP1 (magenta) showing homogeneous G3BP1 signal indicating cells with PC1 condensates are not eliciting an overall stress response (scale bar: 10 µm).