Figure S5.
A scatter plot shows singlets with forward scatter height (FSC-H) on the horizontal axis and forward scatter area (FSC-A) on the vertical axis. A scatter plot shows live cells with side scatter area (SSC-A) on the vertical axis and a viability dye (FVD) on the horizontal axis. A scatter plot shows CD19-positive B cells with side scatter area (SSC-A) on the vertical axis and CD19 on the horizontal axis. A scatter plot shows naïve, unswitched memory (USM), and class-switched memory (CSM) B cells with immunoglobulin D (IgD) on the vertical axis and CD27 on the horizontal axis. A scatter plot shows memory B cells with CD11c on the vertical axis and CD21 on the horizontal axis. A scatter plot shows CD19-positive CD10-negative B cells with immunoglobulin D (IgD) on the vertical axis and CD27 on the horizontal axis. A scatter plot shows CD19-positive B cells with CD10 on the vertical axis and forward scatter height (FSC-H) on the horizontal axis. A scatter plot shows CD19-positive B cells with CD38 on the vertical axis and CD21 on the horizontal axis. A scatter plot shows CD19-positive B cells with CD38 on the vertical axis and CD21 on the horizontal axis. A scatter plot shows double-negative (DN) B cells with CD11c on the vertical axis and CD21 on the horizontal axis. A scatter plot shows naïve B cells with CD11c on the vertical axis and CD21 on the horizontal axis. A scatter plot shows CD19-positive CD10-negative B cells with CD38 on the vertical axis and CD24 on the horizontal axis. A scatter plot shows non-plasmablasts (Non-PB) and plasmablasts (PB) with CD38 on the vertical axis and CD24 on the horizontal axis. A scatter plot shows class-switched (CS) B cells with immunoglobulin M (IgM) on the vertical axis and immunoglobulin D (IgD) on the horizontal axis. A scatter plot shows class-switched (CS) B cells with CD27 on the vertical axis and CD21 on the horizontal axis.
Gating strategy for B cell immunophenotyping by flow cytometry. USM, unswitched memory; CSM, class-switched memory; CS, class-switched; PB, plasmablast; DN, double-negative; FVD, fixable viability dye.